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and consent challenges for multi-dose treatment

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Library preparation For each sample, up to 200 ng of cDNA (as determined by PicoGreen quantification) was fragmented using a Covaris E220 Focused Ultrasonicator (Covaris) with the following parameters: 200 peak incident power, 25% duty cycle, 50 cycles per burst, and 10 s duration repeated 120 times

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Moreover, microbial transplantation studies in germ-free mice with human commensals containing a functional (but not a deletion mutant) microbial cutC gene (responsible for cholineTMA transformation) (23) and genetic manipulation (both gain of function and loss of function) of host hepatic flavin monooxygenase 3 have been shown to modulate host TMAO levels, platelet responsiveness, and thrombosis potential in vivo (24)