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For GSSG determination, the cell extracts were treated with 4-vinyl pyridine (Sigma-Aldrich) for GSH derivatization, and the excess of 4-vinyl pyridine was neutralized with triethanolamine

A novel immunoconjugate compound, L-DOS47, was utilized to increase the pH of an acidic medium in vitro to evaluate its effects on Jurkat, a human T lymphoblast-like cell line

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