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In mouse studies with diet-induced obesity, this led to real weight loss, smaller fat cells, less body fat, lower cholesterol, all without cutting food intake or causing obvious side effects

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This assay is valuable because increased oxidant production results in a diminished GSH/GSSG ratio

The rationale traces back further, to Kraus and colleagues in 2014 ( Nature ), who knocked down NNMT genetically in mice and protected them from diet-induced obesity