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Total GSH was determined in plasma and liver as described by Sedlack & Lindsay ( Reference Sedlak and Lindsay 23 )

This manifested as an increase of intracellular ROS and a marked decrease of GSH

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This is the first report to demonstrate that cellular methylglyoxal accumulates ROS and causes exhausted Glr1 activity with me- thylglyoxal- and ROS-mediated Gpx activity enhancement in C

The most powerful antioxidant the body produces - glutathione - declines with age, chronic stress, and hormonal fluctuation, leaving skin dull, uneven, and reactive while the liver quietly struggles to clear excess estrogen and environmental toxins