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Cell suspensions from left iliac veins and thrombi were prepared, stained with PE mouse anti-rat CD45 (100 g/mL) for 1 h, and fixed, followed by resuspension with 50 L of PBS mixed with 100 L permeabilization medium containing PerCP/Cy5.5 mouse anti rat CD68 (100 g/mL) for 30 min

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However, this process involves multiple transporters, and the specific contribution of OCTN1 is not fully defined

Herzog K, Ijlst L, van Cruchten AG, van Roermund CWT, Kulik W, Wanders RJA, et al

Ionic mutants of Ec CutA1_0SH_S11V/E61V ( Ec 0VV) To examine the thermodynamic parameters of stabilization by ionion interaction at temperatures over 100 C, we constructed several mutant proteins containing substitutions with charged residues, using Ec 0VV as a template