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Nat Rev Microbiol (2017) 15(1):5563

heat-stable -amylase and Na 2 SO 3 were used to analyze NDF ( Rumen sampling and analysis On the last day of the study, approximately four hours after the morning feeding, ruminal fluid (9.8 mL) was collected using a stomach tube ( 2 SO 4 ) in the mobile phase of HPLC