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In preclinical models, the proposed mechanism most often discussed centers on actin sequestration

For Halo-HJURP or -HJURP (8K: F506K, E507K, L509K, L513K, F559K, E560K, I562K, L566K) expression, we generated pBS_Halo-HJURP_PGK1-KI_Hyg R by replacing EGFP of the EGFP-HJURP_PGK1 knock-in vector with Halo and pBS_Halo-HJURP 8K _PGK1-KI_Hyg R by further replacing wild-type HJURP in the Halo-HJURP_PGK1 knock-in vector with HJURP 8K cDNA

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First, air bubbles in the syringe barrel displace liquid volume

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