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Side-effect risk increases with improper dosing, contaminated material, or non-sterile injection technique

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In addition a 100 L of the blood was centrifuged at 10,000XGs for 10 min to isolate the serum and the lactate and NAD + /NADH ratio in the plasma were determined using lactate (Abcam Inc.) and NADH enzymatic kits (Sigma Aldrich) (figure 3B, D)

The external adverse elements, such as cigarette smoking, physical damage, and microbiota influence, may cause keratinocytes damaged through excessive production of ROS(Plenkowska et al., 2020)