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Isolated microvessels from individual mice were plated in Endobasal Medium (Promocell) with 0.4% puromycin for positive selection on coverslips or polyester transwell inserts (Corning)

Here are the steps for this stretch: Start on a mat on your hands and knees

It must only be used under the supervision of a physician or qualified healthcare professional

The measurement markings are more spread out, making it easier to accurately measure small volumes, which is common in peptide research