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Aging 14 (3), 12531264

Xie Z, Yu W, Ye G, Li J, Zheng G, Liu W, et al

52 Insulin secretion is acutely stimulated in response to meals

Culture and purification of SD rat corpus cavernosum endothelial cells by enzymatic digestion combined with mechanical extrusion and fixed-point digestion

This allows researchers to simulate true physiological spikes and observe authentic cell-autonomous recovery periods, eliminating the risks of premature pituitary fatigue or continuous receptor saturation during long-term in vitro assays