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Furnari M, de Bortoli N, Martinucci I, Bodini G, Revelli M, Marabotto E, et al

Bound protein was eluted using a gradient of lysis buffer containing 500 mM imidazole

2008;295:H10512

At the same time, iron-dependent lipoxygenase (LOX) catalyzes polyunsaturated fatty acids (PUFAs) to induce OS, which further alters the permeability of BBB (Iadecola and Anrather 2011)