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The precipitate was collected by centrifugation at 30,000 g for 20 and resuspended in 25 mL buffer B (50 mM MOPS-KOH buffer, pH 7.0, 0.02 mM thiamin pyrophosphate, 1 mM MgCl 2 , 2 mM DTT)

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9 Psychological screening is also important given the high prevalence of mood and other related disorders in patients with PCOS

Three-micron sections were prepared and mounted on slides, followed by deparaffinization and immunofluorescent staining according to a standard protocol as follows: For deparaffinization, the paraffin-embedded sections were placed at 60 C for at least 30 min, incubated twice in xylene (5 minutes each), and then transferred into 100% ethanol (2 minutes), 100% ethanol (2 minutes), 90% ethanol (1 minute), 80% ethanol (1 minute), and 70% ethanol (1 minute) at room temperature

Therefore, it is anticipated that Ficus hirta Vahl can be used to develop natural preservatives for citrus