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8.2, Pharsight Corporation, California, USA) to calculate the pharmacokinetic parameters for each subject, including the bracketed peak concentration (C max ), the area under the blood concentration time curve (AUC 0t and AUC 0 ), the time to peak (T max ), the elimination half-life (t 1/2 ), and the elimination rate constant ()

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Recently, several metabolomics groups have stated that MS/MS multiple reaction monitoring MRMs of reference standard compounds should be done for biomarker verification in LC/MS metabolomics studies (Kitteringham et al

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J Clin Invest 125:665680