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After 14 days, the mice were sacrificed, and their major organs and tissues were analyzed with a hematoxylin and eosin (H&E) stain (Supplementary Fig

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Results Administration of exogenous melatonin alleviates allergic airways disease severity in allergen-exposed mice Given the growing evidence linking circadian disruption to increased severity of allergic asthma ( Alternaria alternata (AA) or PBS daily for four days, with or without concurrent intraperitoneal melatonin treatment using established protocols described previously ( Figure 1A )