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Science 346 , 473477 (2014)

Furthermore, heatmap in Fig

By blocking the activity of NNMT, 5-Amino-1MQ increases the bioavailability of nicotinamide, a precursor to NAD+, thereby elevating intracellular NAD+ levels

Subsequently, the cells were fixed with 4% paraformaldehyde at 16 C for 15 min, permeabilized in phosphate buffered saline (PBS) with 0.1% Triton X-100 for 5 min at 16 C, and incubated with first antibodies at 4 C overnight, followed by incubation with FITC-conjugated secondary antibodies at 16 C for 1 h