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58 Song X, Xu A, Pan W, et al

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The results demonstrated that levels of GSDMD, the terminal substrate in the pyroptosis pathway, positively correlated with the NIHSS score at admission, and that RIPostC treatment significantly reduced serum GSDMD levels in ischemic stroke patients ( in vitro oxygen-glucose deprivation/reoxygenation (OGD/R) model and clinical serum samples ( 3.3.2 Apoptosis Apoptosis is a form of programmed cell death involving endogenous pathways (e.g., mitochondria and endoplasmic reticulum) and exogenous pathways (e.g., death receptors) ( Regarding exogenous pathways, studies have shown that RIPerC exerts neuroprotective effects by upregulating the Bcl-2/Bax ratio through pre-activation of the Notch1 pathway and interaction with the NF-B pathway, thereby reducing cerebral ischemia-reperfusion injury ( 3.3.3 Ferroptosis Ferroptosis is a distinct form of programmed cell death driven by iron-dependent lipid peroxidation

Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the rRNA