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The iron and MDA concentrations were determined using an Iron assay kit (Wuhan Elabscience Biotechnology Co., Ltd.) and a trace MDA assay kit (Beyotime Biotechnology), respectively

Treated groups exhibited synaptic clefts with narrow spacing and sharply defined PSDs, along with presynaptic terminals containing clustered SVs adjacent to membranes

This key effect of GSHee is connected to the findings that GSHee reduced the ability of fentanyl to lower pH, pO 2 and sO 2 while elevating pCO 2 (all consistent with a fall in Ve) and blunted the fentanyl-induced increase A-a-gradient (i.e., impaired gas-exchange in the lungs)

The gut microbiota also plays a significant role in late-phase oral NR metabolism (3 h post-administration), which is converted to NAM by the enzyme BST1, and then deamidated into NA by microbes, allowing PreissHandler synthesis by capable tissues 127