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The XICs for L-Cys and cysteine-aldimine/quinonoid/thiazolidine were analyzed at positive ion TOF with mass of 122.0166 0.0029 Da and 351.010 0.006 Da, respectively

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The click reaction was quenched by adding 500 l of cold acetone (80 C), vortexing and storing overnight at 80 C