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Ginkgo biloba extract protects against methotrexate-induced hepatotoxicity: a computational and pharmacological approach

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FIGURE 7 Identification of a potential EAR-20 binding site on the NMDAR To further investigate the potential binding site of EAR-20 on the NMDA receptor, we performed molecular modelling and docking strategy to predict potential receptor interaction sites

For protein purification, the frozen cell pellets were thawed on ice and resuspended in lysis buffer containing 50 mM Tris-HCl pH 7.0 and 300 mM NaCl supplemented with bovine DNase II, 1 mM PMSF and 5 g ml 1 aprotinin, leupeptin and pepstatin (10 ml per 1-g cell pellet) at room temperature for 30 min