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Selected flavonols targeting cell death pathways in cancer therapy: the latest achievements in research on apoptosis, autophagy, necroptosis, pyroptosis, ferroptosis, and cuproptosis

lower panel, control (c) shows muscle thickness was less increased (H&E, 20

In vitro tumorsphere formation assays Cells are cultivated under serum-free and non-adherent conditions to enrich the cancer stem/progenitor cell population, since only cancer stem/progenitor cells can endure and multiply within such an environment

Mechanistically, Epitalons effect appears to involve a direct interaction with promoter regions of the telomerase gene, where it binds specific DNA sequences (such as ATTTC motifs), potentially modulating transcriptional activity