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The initial models of the SH3arr1-CC complex and Srcarr1-CC complex were built manually by fitting the crystal structures of SH3 (PDB: 2PTK), arr1V2RppFab30Nb32 complex (PDB code: 6NI2) into the experimental electron densities using UCSF Chimera v1.15 65

Bioactivity was also assessed by measuring activation of mGH receptor signal transduction in B16-F10 mouse melanoma cells by phosphorylated STAT5 western blot analysis