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Lysates were clarified by centrifugation (20,000 g, 30 min, 4 C), and protein concentration was determined by BCA assay

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Reduced IGF-I levels are independently associated with glucose intolerance, diabetes, abdominal obesity[309, 310] and atherogenic dyslipidemia[311, 312]

W.DietrichK.et al (2018)

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