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The GABA signal was then isolated by fitting the spectral differences to the edited GABA peak at 3 ppm via nonlinear least-squares fitting using GannetFit, with creatine (Cr) serving as an internal standard for GABA quantification

The efficiency of the extraction was confirmed by the quantification of acetaminophen in roots extracts using either one or the other extraction buffer

Nutr Rev 75(2):7382

This incomplete inactivation mosaicism results in a partially higher gene expression and provides a functional diversity that can enhance immune responses and compensate deleterious mutations