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20 mg blend reconstitution math BAC water 2.0 mL Total concentration 10.0 mg/mL total (5.0 mg/mL each) Per peptide 5.0 mg/mL Common 200 mcg dose 4 units (0.04 mL) BAC water 2.5 mL Total concentration 8.0 mg/mL total (4.0 mg/mL each) Per peptide 4.0 mg/mL Common 200 mcg dose 5 units (0.05 mL) BAC water 3.0 mL Total concentration 6.67 mg/mL total (3.33 mg/mL each) Per peptide 3.33 mg/mL Common 200 mcg dose 6 units (0.06 mL) 3.0 mL is the maximum fill used for this 20 mg blend math

ChemMedChem 14 , 14671483 (2019)

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After TMB substrate solution is added, only those wells that contain Dog TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color