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Am J Physiol Lung Cell Mol Physiol 267:L823L831 Moradi F, Fiocchetti M, Marino M, Moffatt C, Stuart JA (2021) Media composition and O 2 levels determine effects of 17b-estradiol and selective estrogen receptor modulators on mitochondrial bioenergetics and cellular reactive oxygen species

Proponents argue this maintains more stable tissue levels, though others find once-daily dosing equally effective with better compliance

Determination of NADH, NADPH by Fluorescence Lifetime Imaging For the analysis of NADH and NADPH levels, their blue autofluorescence upon UV-excitation can be used