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A nonexclusive alternative explanation for the kinetic patterns with HEDS (and the decreased enzyme activity at high GSH concentrations) could be a Grx(SSCH 2 CH 2 OH) intermediate that is attacked by GSH according to monothiol mechanism (i) from Fig

For instance, the superoxide cannot be detected using DCFDA at all (Kalyanaraman et al

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This phenomenon resulted in glycogen accumulation and glucose uptake, which supported the pentose phosphate pathway to maintain NADPH synthesis, leading to cell survival and growth

3 Ferroptosis: a novel player in depression pathogenesis Dixon and his team introduced the concept of ferroptosis in 2012, characterizing it as an iron-dependent mode of programmed cell death marked by the build-up of lipid hydroperoxides (Dixon et al., 2012)