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Stained plants were transferred into boiling ethanol/glycerin/glacial acetic acid solution (3:1:1) to terminate the staining, then fixed with paraformaldehyde (PFA) solution (2% paraformaldehyde, 0.1% DMSO in PBS) and embedded into 6% agar blocks for sectioning with a vibratome

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Although isoform X1 is absent in certain higher organisms, such as mice, elephants, dogs and chickens, it is highly conserved across many other species ( via the inhibition of NOTCH1 signaling