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Consistent with this, our preclinical work uncovered that TMAO primed renal fibroblasts for conversion to myofibroblasts, the primary collagen-producing cells in the kidney 46 , and contributed to renal fibrosis, a hallmark of kidney damage irrespective of the underlying cause that significantly contributes to eGFR decline 46

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These tests can effectively be used to study the behavior of peptides, their interaction with receptors, and molecular pathways in preclinical laboratory experiments

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Once the nucleus and cytoplasm boundaries were defined for each cell, the mean gray value was computed, representing the average gray value within the selected areas