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Post-treatment, cells were collected and washed 3x with ice-cold PBS, and lysed in 250 l of lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.1% Triton-X-100, 1 mM EDTA, 5 mM MgCl2, 5% glycerol) freshly supplemented with the 100X Halt Protease Inhibitor Cocktail for 20 min on ice

Lipid peroxidation is known to be one of the mo- lecular mechanisms for cell injury in acute mercury poi- soning and is associated with a decrease in cellular anti- oxidants such as glutathione ascorbate, superoxide dis- mutase (SOD) and catalase (CAT) [55,56]

Bio-Rad, Solna, Sweden) and heated to 98 C for 10 min to achieve complete protein denaturation

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Electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT) coatings for implantable deep-brain-stimulating microelectrodes