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showed that Se boosted GPX1 expression by inhibiting DNMT2-mediated DNA methylation of the GPX1 promoter from lessening ROS production in advanced glycation end-product (AGE)-induced heart failure [114]

European J Clin Investig 33(5):370375 Kuo P, Schroeder R (1995) The emerging multifaceted roles of nitric oxide

Cells were seeded in 96-well plates at a density of 5,000 cells (100 l) per well, incubated at 37C for 24 h and then treated with varying concentrations of the test substances (DAPA, Fer-1 and erastin) for specified durations (such as 24, 48 and 72 h)