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The cells were lysed in 25 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1% -mercaptoethanol, 10 mM MgCl 2 , 2.1 mM leupeptin, 1.53 mM aprotinin and 50 U of benzonase (Novagen)

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The maleimide-DAC linker on CJC-1295 achieves the same result by a similar albumin-binding mechanism, extending half-life from 30 minutes to 68 days as reflected in the peptide half-life chart data above

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