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Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min

Animals The study included a sample of 42 male albino Wistar rats, with weights ranging from 180 to 220 g

Half of the kidney was fixed with paraformaldehyde and the other half was frozen with liquid nitrogen

Sperm telomere length is positively associated with the quality of early embryonic development

However, further in vitro assays and preclinical studies with this NP-delivered metal chelator approach are warranted to improve their targeting capacity to the brain as well as to estimate the level of BBB crossing, chelation efficiency and the related toxicity before clinical applications