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All authors contributed to the article and approved the submitted version Acknowledgments The authors thank Servio de Imuno-Hemoterapia from Instituto Portugus de Oncologia de Lisboa Francisco Gentil (IPOLFG EPE) for providing the buffy coats from healthy donors

Biofilm disruption by GSH acidity is triggered through cleaving and destabilization of biopolymers such as extracellular DNA (eDNA), polysaccharides and proteins

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Then, a 1:1 PSM (propensity score matching) with a caliper of 0.2 in R software was used to reduce the influence of confounding factors and the baseline features were also examined for populations after matching