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As mitochondrion is an essential organelle of intracellular redox metabolism for cell survival, the membrane potential of mitochondrion reflects intracellular oxidative damage 37

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Measurement of bound sulfane sulfur A whole brain homogenates were prepared with 9 volumes of ice-cold buffer consisting of 10 mM potassium phosphate (pH 7.4), 1% TritonX-100, 10 mM hydroxylamine, which was used to suppress the activity of PLP-dependent enzymes involved in enzymatic H 2 S production, and protease inhibitor cocktail complete (Roche Diagnostics, Mannheim, Germany) using a Potter type glass homogenizer with a Teflon pestle (1,500 rpm, 10 strokes)

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Similarly, western blot analysis revealed that NRTP significantly increased p-AKT and p-FOXO3A in the M group, and WCV treatment reduced their levels close to those of the control in the VM group, whereas PD-1 antibody treatment did not have a rescue effect on the PM group (Fig