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Oxidative modifications of lipids, proteins, and DNA are induced by mixtures of ascorbic acid and copper or iron for decades [24, 25], contributing to oxidative damage formation by reducing ferric Fe 3+ to ferrous Fe 2+ ions (and Cu 2+ to Cu + ), which in turn can reduce hydrogen peroxide (H 2 O 2 ) to hydroxyl radicals

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EV fractions were obtained through stepwise centrifugation, separating them into large EVs (l-EVs) and small EVs (s-EVs) based on size

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APIs are the therapeutic ingredients that drive the efficacy of pharmaceuticals, nutraceuticals, and cosmeceuticals, making API drug development the cornerstone of modern healthcare