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AML-147 C-MYC Targeting by Degradation: Novel Dual c-Myc/GSPT1 Degrader GT19715 Exerts Profound Cell Kill In Vitro and In Vivo in Acute Myeloid Leukemia and Lymphomas

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RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)

To date, no studies have effectively tested CAPE for CF and, therefore, we intend with this review to elucidate the role of inflammation and oxidative stress for tissue damage seen in CF, associating them with CAPE actions and its pharmacologically active derivatives

Circulation 110 , 705712 (2004)