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97 YangY.WangZ.ZhangL.YinB.LvL.HeJ.et al (2018)

Shewry PR, Halford NG

Cell 157 , 255266 (2014)

ESAT-6, early secretory antigenic target-6

Preparation of genomic DNA for next-generation sequencing and analysis of pooled CRISPR screening Genomic DNA of LDLR high cells and unsorted control cells were extracted respectively using cell lysis buffer (400 mM Sodium chloride, 10 mM Tris, 2 mM EDTA, 0.5% SDS, pH 8) and then subjected to the established procedures 100