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After the washing step, the TMB substrate solution was added to each well followed by incubation at 37 C for 20 min

These remain critically important, but they represent only part of the picture

For the purpose of this invention, the multimerization domain(s) (including small organic scaffold molecules, proteins, protein complexes, polymers, beads, liposomes, micelles, cells) that form a covalent bond with the biologically active proteins can be divided into separate groups, depending on the nature of the reactive group that the one or more multimerization domain(s) contains

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Therapeutic potential of ciclesonide inhalation for COVID-19 pneumonia: report of three cases