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In addition, we note that in response to comments in the FY 2021 IPPS/LTCH PPS final rule (85 FR 59015 through 59016), we increased the burden estimate from 15 hours to 20 hours after consideration of comments stating that additional effort would be necessary to crosswalk discharges to an MS-DRG, specifically if a hospital is not familiar with the MS-DRG classification system, for use in calculating the median payer-specific negotiated charges

To execute this, 200 L of the examined samples, previously diluted with PBS at a 1:5 ratio (v/v), were combined with standard solutions ranging from 0 to 100 mol/L alongside 200 L of blank PBS solution