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Streptomyces strain NEAA-5 formed substrate mycelium and aerial hyphae that transformed into long spiral-shaped spore chains that could be either open or closed

reacts with O-methylisourea in a nucleophilic substitution reaction with the expulsion of methanol to form a guanidino group (has 3 N attached to C, nidi) reacts with fluorodinitrobenzene (FDNB or Sanger's reagent) or trinitrobenzenesulfonate (TNBS, as we saw with the reaction with phosphatidylethanolamine) in a nucleophilic aromatic substitution reaction to form 2,4-DNP-lysine or TNB-lysine

FIGURE 3 3.2.1 -L-glutamyl-4-nitroanilide (GPNA) GPNA, a glutamine analog synthesized by Esslinger, has been widely used to inhibit the glutamine transporter ASCT2 ( in vitro and in vivo (Ma et al., 2021)

59 Lastly, aspirin and curcumin encapsulated in PLGA nanoparticles, combined with sulforaphane, showed synergistic effects in colorectal cancer cell lines SW-480 and HT-29, reducing viability by up to 61%