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Reactions consisted of 10 L assay buffer (50 mM HEPES, 50 mM NaCl, various pH), 10 L enzyme (various concentrations), and 30 L DCF-G (400 M final) diluted in assay buffer

This is likely an important, if not necessary, fundamental change in how we feed ourselves, but it is recognized that this could increase the prevalence of vitamin B12 deficiency because the vitamin is not found in plant-based foods (106, 107)

(2) trans-sulfuration to form GSH and sulfated compounds via homocysteine and cysteine

BMJ Quality & Safety , 9 (1), 6-13