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doi: 10.2307/4444260 46 SolainiGSgarbiGBaraccaA

doi: 10.1038/s41580-023-00648-1, 47 GaoZ.WangG.ChenY.YuanW.CaiJ.FengA.et al

The oligonucleotide anti-tag can be formed in a variety of ways

The three libraries were generated using error-prone PCR as described above, using the following conditions to produce varying levels of mutagenesis: Library 1: 2 M 8-oxo-dGTP, 2 M dPTP, 20 PCR cycles Library 2: 4 M 8-oxo-dGTP, 1 M dPTP, 20 PCR cycles Library 3: 8 M 8-oxo-dGTP, 0.5 M dPTP, 20 PCR cycles The library sizes (approximated by number of transformants as described above) were 6 10 7 for each library