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Once a vial is reconstituted and the dose is drawn into the syringe, the location and technique of the injection determine whether the material is delivered into the intended tissue layer, how comfortable the process is, and whether the skin at the injection site stays healthy over a multi-week protocol

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In contrast, M1 polarization resulted in a significant increase in the secretion of the pro-inflammatory cytokine TNF, as well as elevated mRNA levels of TNF, IL1, and the hypoxia-inducible factor HIF1, confirming a pro-inflammatory phenotype (Fig