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The abovementioned tube-induction medium was added to each well for a total of 120 l (96-well plates) or 1 ml (24-well plates) at cell densities of 05106 cells/ml for cell viability analysis or collecting 3D culture supernatants derived from Lu99 cells

[DOI] [PubMed] [Google Scholar] 147.Ricci S., Businaro R., Ippoliti F., Lo Vasco V.R., Massoni F., Onofri E., Troili G.M., Pontecorvi V., Morelli M., Rapp Ricciardi M., et al

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Others prescribe continuously for 8 to 12 weeks