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The identification of the first GPER1 specific agonist G1 was described in 2006 ( The HSI index serves as a simple and efficient screening tool for MASLD ( de novo lipogenesis, as shown by the downregulation of FASN, ACC1, and SCD1, leading to a significant reduction in TG accumulation and lipid droplets

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Once the stage reached 40 C, the droplet was warmed at a rate of 10 C per min 1 to 5 C where it remained at 5 C for 1 min to ensure complete thawing