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Rhonda: And what ends up happening is the UCP1 totally takes that away

33 L-dopa-associated S-adenosylmethionine depletion

In brief, after labeling and washing, cells were lysed with radioimmunoprecipitation assay (RIPA) lysis buffer supplemented with 1 protease inhibitor cocktail (Thermo Scientific, 78429)

Quantitative profiling using HPLC-MS/MS is highly reliable when employing an isotope dilution approach, wherein stable isotope-labeled internal standards (IS) effectively compensate for matrix effects (examples are shown for GSH and Glu in Fig

Interestingly, the most striking respiratory difference attributable to loss of GSH emerged upon treatment of Gclc fl/fl and Gclc fl/fl Mb1-Cre + FoB with the mitochondrial ionophore FCCP, which increases the OCR to the maximal respiration (i.e