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Using a non-USP-grade water source for peptide reconstitution risks variable composition, contamination, and interference with downstream assays

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Vitamin B12 is a nutrient that offers numerous benefits to the body, including: Helping make your blood cells healthy Helping with nerve cell function Making DNA, the genetic material in all of your cells Helping prevent megaloblastic anemia, a blood condition that makes people tired and weak Some groups are at heightened risk of B12 deficiency, including: Older adults Vegans Vegetarians People with digestive issues, such as celiac disease or Crohn's disease People who have had stomach or intestinal surgery If a B12 deficiency goes untreated, it can cause physical, neurological, and psychological problems

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Cryopreservation The DMSO and ZIL solutions were prepared by mixing with ultrapure water or DMEM/FBS