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Cell line generation ex vivo Three tumors for each treatment group were excised, placed in Petri dishes with DMEM/F12 medium, cut into fragments with a sterile scalpel, and subjected to digestion with 1 mg/ml collagenase type IV (Sigma-Aldrich) for 30 min at 37 C under aseptic conditions

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Furthermore, GO and KEGG transcriptomic analyses of DEGs between the KS and NC groups in human similarly demonstrated significant suppression of key lipid-related pathways as shown in Fig

This boost in energy can be particularly beneficial for those following calorie-restricted diets