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Third, we have shown that the acs1 mutant is hypersensitive to fluconazole in vitro and that deletion of ACS1 increases the efficacy of fluconazole during mammalian infection 23

O-carboxymethylated chitosan can occur in an ice bath or at room temperature if sodium hydroxide and monochloroacetic acid are added, along with isopropanol/water as a solvent (Jayakumar et al., 2010)