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Unlike small molecules that may lack selectivity, or large proteins that face delivery challenges, peptides often provide an optimal balance of specificity, potency, and manufacturability

For sandwich ELISA, Maxisorp plates were coated overnight at 4 C with a polyclonal goat anti-HSA antibody (Sigma, # A-7544) diluted 1:1000 in PBS for the unfused HSAs or with monoclonal rabbit anti-camelid VHH antibody (Genscript, # A01860) diluted 1:1000 in PBS for the Albu-LiTE fusion proteins

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This means a certain amount of TB-500 is needed to physically bind a certain amount of Actin to achieve the effect

We probably, therapeutically, are using close to 250 over the years, which is obviously tiny compared to that